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Evaluation of the effect of Emdogain and Simvastatin on periodental ligament stem cells differentiation to osteoblasts, in vitro

Background & aims:
One of the primary characteristics of periodontium, regenerating periodontal structure in response to destructive factors, can be done by cells which differentiate into cells or tissues very similar to the periodontium. These cells are stem cells which have an important role in maintainance of tissue homeostasis. The aim of this study was evaluating human PDL stem cell differentiation to osteoblast in standard osteogenic medium and comparing the results with cells cultured in osteogenic medium supplemented with Emdogain and Simvastatin.
Materials and methods:
After extraction of teeth under sterile condition, the periodontal ligament tissues attached to the middle third of the root surface were removed. Cells expanded in the culture and at the 2nd passage, cells in the developing adherent layer were used for osteogenic differentiation and adding the test materials. Osteogenic differentiation was evaluated by Alizarin Red Staining, Alkaline Phosphatase (ALP) activity and calcium content tests.
Results:
Quantitative analysis of ARS demonstrated that mineralized nodule formation was highest in the EMD+DEX group. Results from ALP activity test on 7th day, demonstrated that the maximum amount of the test was in group with both dexamethasone and statin. On the 14th day, results of the test showed that the maximum amount of ALP activity was in EMD group. The amount of calcium in the control group was lowest and in EMD+DEX group was highest on all the tested days.
Conclusion:
The effect of 100 µg/ml emdogain on osteogenic differentiation of human PDL stem cells is considerable and 10-8 M simvastatin has an effect comparable to 10-8 dexamethasone.


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-:  سارا سهیلی فر
موضوع:  پریو
Level:  Undergraduate

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